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KMID : 0923620100100050145
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Immune Network
2010 Volume.10 No. 5 p.145 ~ p.152
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Flowers of Inula japonica Attenuate Inflammatory Responses
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Choi Jeon-Hyeun
Park Young-Na
Li Ying
Jin Mei-Hua
Lee Ji-ean
Lee Youn-Ju
Son Jong-Keun
Chang Hyeun-Wook
Lee Eun-kyung
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Abstract
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Background
The flowers of Inula japonica (Inulae Flos) have long been used in traditional medicine for the treatment of inflammatory diseases. In the present study, we investigated the anti-inflammatory properties of Inulae Flos Extract (IFE).
Methods
The anti-inflammatory effects of IFE against nitric oxide (NO), PGE2, TNF-α, and IL-6 release, as well as NF-κB and MAP kinase activation were evaluated in RAW 264.7 cells.
Results
IFE inhibited the production of NO and the expression of inducible nitric oxide synthase (iNOS) in LPS-stimulated RAW264.7 cells. In addition, IFE reduced the release of pro-inflammatory cytokines, such as TNF-α and IL-6. Furthermore, IFE inhibited the NF-κB activation induced by LPS, which was associated with the abrogation of IκB-α degradation and subsequent decreases in nuclear p65 and p50 levels. Moreover, the phosphorylation of ERK, JNK, and p38 MAP kinases in LPS-stimulated RAW 264.7 cells was suppressed by IFE in a dose-dependent manner.
Conclusion
These results suggest that the anti-inflammation activities of IFE might be attributed to the inhibition of NO, iNOS and cytokine expression through the down-regulation of NF-κB activation via suppression of IκBα and MAP kinase phosphorylation in macrophages.
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KEYWORD
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Inula japonica, Inulae Flos Extract (IFE), Nitric oxide (NO), iNOS, Cytokine, NF-κB, MAP kinase
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